Annals of Maxillofacial Surgery

REVIEW ARTICLE
Year
: 2019  |  Volume : 9  |  Issue : 1  |  Page : 168--173

Maxillary fungal osteomyelitis: A review of literature and report of a rare case


Ankita Srivastava1, Mounabati Mohpatra1, Ashoka Mahapatra2,  
1 Department of Dentistry, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India
2 Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India

Correspondence Address:
Dr. Ashoka Mahapatra
Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar - 751 019, Odisha
India

Abstract

Fungal osteomyelitis is a life-threatening and seldom seen opportunistic infection. It is commonly an affectation of the nose and paranasal sinuses within the orofacial region. It is an aggressive infection that needs to be addressed promptly to prevent fatal consequences. Here, we present a case of a 62-year-old female who presented with complaints of pain and pus discharge from the extraction socket of the left maxillary 23, 24, 25, 26 teeth. She had a history of uncontrolled diabetes mellitus. On further investigation, using diagnostic and Interventional aids, a final diagnosis of maxillary fungal osteomyelitis was made. The infective fungal agents were a mixture of Mucorales and Aspergillus species. A review of all literatures on the subject in the past 13 years using different search engines showed that craniofacial fungal infections with primary maxillary involvement are a rare phenomenon. The primary aim of reporting this case, therefore, is to highlight its rarity, presentation, management and most importantly the outcome of management.



How to cite this article:
Srivastava A, Mohpatra M, Mahapatra A. Maxillary fungal osteomyelitis: A review of literature and report of a rare case.Ann Maxillofac Surg 2019;9:168-173


How to cite this URL:
Srivastava A, Mohpatra M, Mahapatra A. Maxillary fungal osteomyelitis: A review of literature and report of a rare case. Ann Maxillofac Surg [serial online] 2019 [cited 2019 Dec 14 ];9:168-173
Available from: http://www.amsjournal.com/text.asp?2019/9/1/168/260333


Full Text



 Introduction



Concomitant fungal infection of mucormycosis and aspergillosis is a rare, invasive, rapidly progressive, and life-threatening fungal disease of the maxillofacial region.[1] There is a paucity of incidence of such combination opportunistic infections within the maxillofacial region as a result of the rich vascularity of this specific anatomy. Fungal organisms such as Mucorales and Aspergillus, however, can evade this defense mechanism in view of their potential virulence.[2] This is more common among patients with a depressed immune system or with immunocompromised clinical states. Attributable risk factors for such infections are: uncontrolled diabetes mellitus; long-term steroid therapy; hematological conditions like leukemia and lymphomas; and renal failure and Acquired Immune Deficiency Syndrome (AIDS).[3] These fungi gain entry into the body through different portals such as the nose, breached skin, and tooth extraction sockets. Pulmonary affectation and rhino-orbito-cerebral involvement are the most common form of mucormycosis. However, other primary sites of infection include the skin, ears, gastrointestinal tract and there could be disseminated forms involving multiple sites.[1]

According to published literatures, mortality rates of mucormycosis ranges from 10% to 100% depending on the site of infection and underlying predisposing factors.[2] Maxillary sinus mucormycosis have a poor prognosis and high mortality rate (46%).[4] Aspergillosis has a mortality rate of 30% with a poor prognosis in pediatric cases (with a mortality rate as high as 85%).[5] Early diagnosis and immediate intervention are essential for such patients. Treatment modality includes control of the underlying risk factors, antifungal therapy, surgical debridement, supportive therapy, and surgical or prosthetic rehabilitation (RECONSTRUCTION) is very important in view of the restoration of quality of life to the premorbid state.

We present a review and our experience of concomitant Mucorales and Aspergillus infection of the maxilla causing extensive necrosis in a female with uncontrolled diabetes who had a history of tooth extraction. Through this article, it is our effort to bring awareness among clinicians regarding such a rare but potentially fatal fungal infection.

 Materials and Methods



We did the literature search to analyze the site specificity and types of immunocompromised conditions that may be associated with a rare concomitant occurrence of mucormycosis and aspergillosis. A database search in PubMed, ScienceDirect, EBSCO, Medline, and Google Scholar was carried out using MeSH terms such as “mucormycosis,” “concomitant infection,” “aspergillosis,” “fungal infection,” and “orofacial.” Only reports of concomitant infection of Mucorales and Aspergillus were included in the review. Reports with other fungal infection were excluded. All articles published in English were included in this analysis.

 Results



We reviewed 35 literatures on the subject (orofacial fungal infection) over the past 13 years (2005–2018). Using the inclusion and exclusion criteria, 14 relevant literatures were included. Of the total 14 literatures involving mixed fungal infections of both Mucorales and Aspergillus species, there were 12 case reports and 2 case series (2 + 2) making a total of 16 cases. Out of these, 15 cases had underlying debilitating disease or immunocompromised condition according to the following distribution; uncontrolled diabetes mellitus (n = 6, 40%); leukemia (n = 3, 20%); postrenal transplant infection (n = 2, 13.3%). Other conditions were Castleman disease, lymphomas, and tetralogy of Fallot [Diagram 1]. The paranasal sinuses were the most common sites of involvement in this review (n = 14, 87.5%). None of the cases showed primary involvement of maxilla [Table 1], but in our case, maxilla was primarily involved most likely through the extraction socket.[INLINE:1]{Table 1}

 Case Report



A 62-year-old female patient reported to the Dental Surgery Outpatient Department (OPD) of All India Institute of Medical Sciences Bhubaneswar, Odisha, India, with a chief complaint of pain, pus discharge, and diffuse swelling of the left midfacial region following the extraction of teeth from the left maxillary quadrant. The extraction was performed 2 months before this visit. She was a known case of type II diabetes mellitus, and the exodontia was performed by a general dental practitioner. Her past dental records revealed that she had chronic generalized periodontitis with high fasting blood sugar (FBS) and postprandial blood sugar (PPBS (i.e., 139 mg/dl and 193 mg/dl, respectively) at the time of exodontia. Few days following the exodontia of the left upper quadrant (21–26), she developed regurgitation of water and other oral fluids through the left nostril along with nasal congestion which was also later associated with pain and discomfort in the same region for which she reported to our OPD.

On clinical examination, the patient was of average built and was in obvious painful distress. Extraoral examination revealed diffuse swelling on the left side of the midface, extending anteroposteriorly from the nasolabial fold to the left tragal region and superoinferiorly from infraorbital rim to the level of the oral commissure. The overlying skin appeared to be smooth, shiny, and mildly erythematous and it was soft and tender to digital palpation. Intraoral examination revealed an exposed necrotic alveolar bone in the area from 23 to 26 tooth sockets [Figure 1]. The surrounding labial and palatal mucosa were inflamed, edematous, with irregular borders. Foul smelling pus oozed from the extracted teeth sockets.{Figure 1}

The panoramic view revealed a moth-eaten appearance of the left maxillary alveolar bone and haziness of the maxillary antrum of the same side. Noncontrast computerized tomographic scan of the craniomaxillofacial region revealed osteolytic lesions involving the left maxillary alveolar bone and maxillary antrum [Figure 2] and [Figure 3]. Based on clinical and radiological findings, a provisional diagnosis of osteomyelitis of the left maxilla was made presuming to be of bacterial origin. A pus sample was appropriately taken and sent to the microbiology department for routine culture and sensitivity test.{Figure 2}{Figure 3}

The patient was hospitalized and planned for sequestrectomy and saucerization of the affected region under general anesthesia. Before the intervention, routine biochemical, serological, and hematological examinations were done. All test reports appeared within normal limits except a significantly elevated FBS and PPBS levels (230 and 450 mg/dl, respectively). These were monitored and controlled in consultation with the endocrinologist by initiating appropriate insulin regime.

Antibiotic prophylactic regime was initiated including intravenous (IV) injections of Monocef 1 g (Aristo India) at 12 h interval and Metrogyl 500 mg (Unichem India) at 8 h interval. Under general anesthesia and total aseptic condition, sequestrectomy and debridement of necrotic bone were done through the intraoral approach [Figure 4]. The excised hard and soft-tissue specimen was put in 10% formal saline and sterile distilled water and sent for histopathological and microbiological examination, respectively. The microbiological examination involved screening for fungal hyphae using the Gomori methenamine silver (GMS) staining technique. The postoperative period was uneventful. The antibiotic regime was continued for 5 days with regular twice daily dressings.{Figure 4}

Microbiological examination

The aspirated pus sent for culture before surgery was sterile. The tissue was homogenized under aseptic condition and subjected to bacterial (aerobic and anaerobic), tubercular, fungal culture, Gram-stain, Ziehl–Neelsen (ZN) stain, and potassium hydroxide (KOH) mount examination. Gram-stain revealed good number of pus cells and no bacteria. ZN smear was also negative for acid-fast bacilli. However, KOH preparation showed hyaline broad nonseptate hyphae. The bacterial and tubercular culture reports were sterile. Fungal culture report showed grayish white, cotton-like floppy growth on the 7th incubation day. By lactophenol cotton blue mount from the growth, it was identified to be Mucor species. A repeat fungal culture from the same sample was undertaken to rule out contamination. However, a mixed growth of Mucor and Aspergillus spp. was obtained on the 8th day.

Histopathological examination

Histopathology of the tissue under special stains such as hematoxylin and eosin (H and E), Periodic acid–Schiff (PAS), (GMS) highlighted the presence of broad nonseptate hyphae and acute angle branching septate hyphae and yeast forms [Figure 5], [Figure 6], [Figure 7]. The features were consistent for the fungal invasion of bone with Mucor and Aspergillus species.{Figure 5}{Figure 6}{Figure 7}

Final diagnosis

Taking into account the microbiological and histopathological evidence, a diagnosis of fungal osteomyelitis secondary to Mucor and Aspergillus species tissue invasion was made.

Thereafter, antifungal treatment of IV amphotericin B (1 mg/kg/day) and oral voriconazole (200 mg 12 hourly) was started with a monitoring of renal function test, liver function test, complete blood count, and blood sugar level. After 8 doses of amphotericin-B infusions, the patient developed neutrophilic leukocytosis, hence amphotericin B was stopped, and oral voriconazole was continued for 12 weeks. The patient was given an obturator and was kept on follow-up. Postoperative healing was satisfactory. There was no recurrence of disease after 2 years of follow-up [Figure 8].{Figure 8}

 Discussion



Aspergillosis and mucormycosis of the orofacial region are rare, but aggressive, opportunistic infections which may lead to fatal consequences.[20] They belong to the group of Aspergilli and Zygomycetes, respectively. The most common species forms associated with mucormycosis are Rhizomucor, Rhizopus, and Absidia.[21] Mucormycosis is the third most prevalent opportunistic fungal infection after candidiasis and aspergillosis.[22] Paul Tauf in 1885 reported the first case of human mucormycosis.[23] Scheld had reported a case of simultaneous disseminated aspergillosis and zygomycosis in a leukemic patient in 1979.[24] Most common sites for the manifestation of mucormycosis infection are the nasal cavity, orbit, and cerebral tissues.[25] Other systemic sites of involvement are pulmonary, gastrointestinal, cutaneous, and disseminated forms.[4]

In the literature review by Chermetz et al., 51 cases were reported to have a concomitant infection with Aspergillus and Mucorales of which only 9 cases had sino-orbito-oral involvement. The remaining 42 cases had other systemic involvement, particularly of the skin and lungs.[5]

Immunocompromised individuals are more vulnerable to such opportunistic infections.[26] Commonly involved predisposing factors are uncontrolled diabetes, lymphomas, leukemia, renal failure, immunosuppressive or long-term steroid therapy, organ transplant, renal failure, protein-energy malnutrition, and AIDS.[27],[28] Rarely, does it involve healthy individuals.[29] Recipients with bone marrow transplant are exclusively susceptible to infection with an incidence of 5%–14%.[30] Attributable risk factor in our case is uncontrolled diabetes mellitus. The common portal of entry for pathogens is nose and paranasal sinuses through inhalation or breached skin and mucous membrane. An uncommon, but invasive portal site is the dental extraction socket.[31] The portal of entry in this case could be through the extracted tooth socket due to extraneous contamination. Vascular invasion is the key pathophysiological feature of human Mucorales infection.[32] Spores or vegetative forms of Mucorales invade the arteries and form a thrombus within the vessel resulting in ischemic infarcts and subsequently necrosis of regional hard and soft tissues.[2],[26],[32] The destruction of tissues as a result of Aspergillus infection is due to the action of inflammatory cells recruited to the affected site.[5] High glucose levels, acidic environment, low oxygen, and high iron levels facilitate germination and growth of fungal spores.[33]

In case of diabetes mellitus, primary contributing factors are deranged granulocyte-phagocytic ability and altered polymorphonuclear leukocyte response.[34] Second contributing factor is microangiopathy and atherosclerosis (peripheral vascular diseases), resulting in local tissue ischemia and increased vulnerability to infection.[35] Cases of diabetic ketoacidosis are at higher risk of developing mucormycosis due to the elevation of serum iron. Moreover, fungal hyphae produce “rhizoferrin” which binds to the available serum iron and forms iron-rhizoferin complexes. This is the nutrient element for growth, development, and multiplication of Mucorales spores.[36]

In orofacial region, clinical manifestation of mucormycosis and aspergillosis are rhinorrhea, facial cellulitis, nasal discharge, and turbinate necrosis along with lethargy fever and headache.[37] Ophthalmic involvement occurs in later stages and includes painful eyes, blurred vision, conjunctival suffusion, ptosis, proptosis, and chemosis.[38] Loss of vision has also been reported indicating retinal artery thrombosis.[33] Our case did not show any ophthalmic involvement.

According to the joint clinical guidelines by the European Confederation of Medical Mycology, direct microscopy, culture, and histopathology are recommended diagnostic aids in the management of this disease entity.[39] Further advanced techniques used in the diagnosis of Aspergillus infection include detection of galactomannan, beta-D-glucan and DNA by Polymerase Chain Reaction-based methods.[40] According to the Infectious Disease Society of America, detection of galactomannan, beta-D-glucan and various stains like H and E, PAS, Grocott-GMS nitrate and fluorescent stains are frequently used to reveal characteristic hyphae content in tissues.[20]

Radiographic techniques serve as a supportive diagnostic aid rather than being a definitive diagnostic modality. Radiographic picture of the affected area may show erosion of underlying bone, mucosal thickening, or opacification of sinus cavity.

Treatment includes sequestrectomy with proper wound debridement. Antifungal drug regime includes amphotericin B, voriconazole and posaconazole. Postdebridement defect if created can be rehabilitated by soft tissue and hard tissue reconstruction or by providing an obturator for the patients.[41]

 Conclusion



Immunocompromised patients are vulnerable for fatal fungal infections involving bones and soft tissues of the oral cavity. Such infections always create a dilemma for the clinicians to reach a definitive diagnosis due to paucity of occurrence and reporting. Early diagnosis and treatment can save such consequences. The possibility of such kind of fungal osteomyelitis should be taken into account as a differential diagnosis for similar clinical situations.

Acknowledgment

We need to acknowledge Mr. Purusottam Swain Incharge of medical record division for providing the patient's file for the preparation of this report.

Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

References

1Verma A, Singh V, Jindal N, Yadav S. Necrosis of maxilla, nasal, and frontal bone secondary to extensive rhino-cerebral mucormycosis. Natl J Maxillofac Surg 2013;4:249-51.
2Spellberg B, Edwards J Jr., Ibrahim A. Novel perspectives on mucormycosis: Pathophysiology, presentation, and management. Clin Microbiol Rev 2005;18:556-69.
3Leitner C, Hoffmann J, Zerfowski M, Reinert S. Mucormycosis: Necrotizing soft tissue lesion of the face. J Oral Maxillofac Surg 2003;61:1354-8.
4Roden MM, Zaoutis TE, Buchanan WL, Knudsen TA, Sarkisova TA, Schaufele RL, et al. Epidemiology and outcome of zygomycosis: A review of 929 reported cases. Clin Infect Dis 2005;41:634-53.
5Chermetz M, Gobbo M, Rupel K, Ottaviani G, Tirelli G, Bussani R, et al. Combined orofacial aspergillosis and mucormycosis: Fatal complication of a recurrent paediatric glioma-case report and review of literature. Mycopathologia 2016;181:723-33.
6Maiorano E, Favia G, Capodiferro S, Montagna MT, Lo Muzio L. Combined mucormycosis and aspergillosis of the oro-sinonasal region in a patient affected by Castleman disease. Virchows Arch 2005;446:28-33.
7Lador N, Polacheck I, Gural A, Sanatski E, Garfunkel A. A trifungal infection of the mandible: Case report and literature review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:451-6.
8Alfano C, Chiummariello S, Dessy LA, Bistoni G, Scuderi N. Combined mucormycosis and aspergillosis of the rhinocerebral region. In Vivo 2006;20:311-5.
9Pellacchia V, Terenzi V, Moricca LM, Buonaccorsi S, Indrizzi E, Fini G. Brain abscess by mycotic and bacterial infection in a diabetic patient: Clinical report and review of literature. J Craniofac Surg 2006;17:578-84.
10Chua JL, Cullen JF. Fungal pan-sinusitis with severe visual loss in uncontrolled diabetes. Ann Acad Med Singapore 2008;37:964-7.
11Kishel JJ, Sivik J. Breakthrough invasive fungal infection in an immunocompromised host while on posaconazole prophylaxis: An omission in patient counseling and follow-up. J Oncol Pharm Pract 2008;14:189-93.
12Suwan Y, Punyawattanaporn A, Preechawai P. Rhino-orbital fungal infection: Two cases report. J Med Assoc Thai 2012;95:739-42.
13Vaidya D, Shah P. Coinfection by Aspergillus and zygomycetes species in a case of acute rhinosinusitis. Case Rep Otolaryngol 2011;2011:382473.
14Shashir W, Rupali S, Sujata L, Preeti M. Concomitant zygomycosis and aspergillosis of the rhinocerebral region in a post renal transplant patient. Indian J Transplant 2014;8:25-7.
15Davoudi S, Anderlini P, Fuller GN, Kontoyiannis DP. A long-term survivor of disseminated Aspergillus and Mucorales infection: An instructive case. Mycopathologia 2014;178:465-70.
16Nitin MN, Hitesh V, Rps P. Co-existing mucormycosis with aspergillosis in a patient with diabetes mellitus- first case report. Online J Otolaryngol 2014;4:257-64.
17Mahomed S, Basanth S, Mlisana K. The successful use of amphotericin B followed by oral posaconazole in a rare case of invasive fungal sinusitis caused by co-infection with mucormycosis and Aspergillus. IDCases 2015;2:116-7.
18Goswami S, Vohra R, Raju BM, Agarwal A. Concomitant mucormycosis and aspergillosis of rhinocerebral region in a renal transplant patient-air cooler being the culprit. Indian J Med Case Rep 2016;5:30-4.
19Habroosh FA, Eatamadi H, Mohamed RM. Concomitant orbital aspergillosis and mucormycosis in a 17 months old immunocompetent child. Saudi J Ophthalmol 2017;31:193-5.
20Choudhary P, Bhargava D, Sharma R. Mucormycosis of maxilla. Indian J Dent Adv 2014;6:1503-7.
21Auluck A. Maxillary necrosis by mucormycosis. A case report and literature review. Med Oral Patol Oral Cir Bucal 2007;12:E360-4.
22Karanth M, Taniere P, Barraclough J, Murray JA. A rare presentation of zygomycosis (mucormycosis) and review of the literature. J Clin Pathol 2005;58:879-81.
23Dave SP, Vivero RJ, Roy S. Facial cutaneous mucormycosis in a full-term infant. Arch Otolaryngol Head Neck Surg 2008;134:206-9.
24Scheld WM, Royston D, Harding SA, Hess CE, Sande MA. Simultaneous disseminated aspergillosis and zygomycosis in a leukemic patient. South Med J 1979;72:1325-8.
25Selvamani M, Donoghue M, Bharani S, Madhushankari GS. Mucormycosis causing maxillary osteomyelitis. J Nat Sci Biol Med 2015;6:456-9.
26Pogrel MA, Miller CE. A case of maxillary necrosis. J Oral Maxillofac Surg 2003;61:489-93.
27Salisbury PL 3rd, Caloss R Jr., Cruz JM, Powell BL, Cole R, Kohut RI, et al. Mucormycosis of the mandible after dental extractions in a patient with acute myelogenous leukemia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;83:340-4.
28McNulty JS. Rhinocerebral mucormycosis: Predisposing factors. Laryngoscope 1982;92:1140-3.
29Del Valle Zapico A, Rubio Suárez A, Mellado Encinas P, Morales Angulo C, Cabrera Pozuelo E. Mucormycosis of the sphenoid sinus in an otherwise healthy patient. Case report and literature review. J Laryngol Otol 1996;110:471-3.
30Fukuda T, Boeckh M, Carter RA, Sandmaier BM, Maris MB, Maloney DG, et al. Risks and outcomes of invasive fungal infections in recipients of allogeneic hematopoietic stem cell transplants after nonmyeloablative conditioning. Blood 2003;102:827-33.
31Liu M, Spellberg B, Phan QT, Fu Y, Fu Y, Lee AS, et al. The endothelial cell receptor GRP78 is required for mucormycosis pathogenesis in diabetic mice. J Clin Invest 2010;120:1914-24.
32Fogarty C, Regennitter F, Viozzi CF. Invasive fungal infection of the maxilla following dental extractions in a patient with chronic obstructive pulmonary disease. J Can Dent Assoc 2006;72:149-52.
33Kheirkhah L, Asoubar S, Abdi AH, Mahmoudi A. Rhinocerebral mucormycosis in a patient with diabetes type 1 presenting as ptosis and facial palsy report from alborz hospital of Karaj from Iran. Int Clin Pathol J 2017;5:211-3.
34Jones AC, Bentsen TY, Freedman PD. Mucormycosis of the oral cavity. Oral Surg Oral Med Oral Pathol 1993;75:455-60.
35Tugsel Z, Sezer B, Akalin T. Facial swelling and palatal ulceration in a diabetic patient. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;98:630-6.
36Dökmetaş HS, Canbay E, Yilmaz S, Elaldi N, Topalkara A, Oztoprak I, et al. Diabetic ketoacidosis and rhino-orbital mucormycosis. Diabetes Res Clin Pract 2002;57:139-42.
37Buhl MR, Joseph TP, Snelling BE, Buhl L. Temporofacial zygomycosis in a pregnant woman. Infection 1992;20:230-2.
38Harril WC, Stewart MG, Lee AG, Cernoch P. Chronic rhinocerebral mucormycosis. Laryngoscope 1996;106:1292-7.
39Cornely OA, Arikan-Akdagli S, Dannaoui E, Groll AH, Lagrou K, Chakrabarti A, et al. ESCMID and ECMM joint clinical guidelines for the diagnosis and management of mucormycosis 2013. Clin Microbiol Infect 2014;20 Suppl 3:5-26.
40Ambasta A, Carson J, Church DL. The use of biomarkers and molecular methods for the earlier diagnosis of invasive aspergillosis in immunocompromised patients. Med Mycol 2015;53:531-57.
41Rai S, Misra D, Misra A, Jain A, Jain P, Dhawan A. Palatal mucormycosis masquerading as bacterial and fungal osteomyelitis: A rare case report. Contemp Clin Dent 2018;9:309-13.