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Year : 2013  |  Volume : 3  |  Issue : 2  |  Page : 114-125

GMP-level adipose stem cells combined with computer-aided manufacturing to reconstruct mandibular ameloblastoma resection defects: Experience with three cases

1 Institute of Biomedical Technology, University of Tampere; Department of Eye, Ear and Oral Diseases, Tampere University Hospital, Tampere, Finland
2 Institute of Biomedical Technology, University of Tampere, Tampere; Department of Oral and Maxillofacial Surgery, University of Oulu, Oulu, Finland
3 Department of Eye, Ear and Oral Diseases, Tampere University Hospital, Tampere, Finland
4 Department of Oral and Maxillofacial Surgery, Central Hospital of Central Finland Health Care District, Jyväskylä, Finland
5 Institute of Biomedical Technology, University of Tampere, Tampere, Finland

Correspondence Address:
George K Sándor
Institute of Biomedical Technology, University of Tampere, Biokatu 12 Krs 6, 33520 Tampere
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Source of Support: The Finnish Funding Agency for Technology and Innovation (TEKES) and the Competitive Research Funding of Tampere University Hospital

DOI: 10.4103/2231-0746.119216

PMID: 24205470

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Background: The current management of large mandibular resection defects involves harvesting of autogenous bone grafts and repeated bending of generic reconstruction plates. However, the major disadvantage of harvesting large autogenous bone grafts is donor site morbidity and the major drawback of repeated reconstruction plate bending is plate fracture and difficulty in reproducing complex facial contours. The aim of this study was to describe reconstruction of three mandibular ameloblastoma resection defects using tissue engineered constructs of beta-tricalcium phosphate (β-TCP) granules, recombinant human bone morphogenetic protein-2 (rhBMP-2), and Good Manufacturing Practice (GMP) level autologous adipose stem cells (ASCs) with progressively increasing usage of computer-aided manufacturing (CAM) technology. Materials and Methods: Patients' three-dimensional (3D) images were used in three consecutive patients to plan and reverse-engineer patient-specific saw guides and reconstruction plates using computer-aided additive manufacturing. Adipose tissue was harvested from the anterior abdominal walls of three patients before resection. ASCs were expanded ex vivo over 3 weeks and seeded onto a β-TCP scaffold with rhBMP-2. Constructs were implanted into patient resection defects together with rapid prototyped reconstruction plates. Results: All three cases used one step in situ bone formation without the need for an ectopic bone formation step or vascularized flaps. In two of the three patients, dental implants were placed 10 and 14 months following reconstruction, allowing harvesting of bone cores from the regenerated mandibular defects. Histological examination and in vitro analysis of cell viability and cell surface markers were performed and prosthodontic rehabilitation was completed. Discussion: Constructs with ASCs, β-TCP scaffolds, and rhBMP-2 can be used to reconstruct a variety of large mandibular defects, together with rapid prototyped reconstruction hardware which supports placement of dental implants.

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